Binary vector pcambia-1300

WebpCambia1300 Plant Expression Vector General notes Vector contains kanamycin resistance gene for bacterial selection, hygromycin B resistance gene for plant selection and no reporter gene to allow construction of user's own system. The pCambia1300 vector offers: High copy number in E.coli for high DNA yields WebpCAMBIA1300 Agrobacterium binary vector for plant transformation, with hygromycin- and kanamycin-resistance genes. Sequence Author: Cambia Open in SnapGene Try …

质粒载体pWM1011,pCM184,pGWB403系列 - Biovector

WebNov 15, 2024 · The pCambia-1300 binary vector was used as a backbone. A pCambia–BSCTV vector was used as template to amplify pV86 or pC86 trans-activated promoters using primers pV86-GUS-R1-F/R or pC86 … WebA. tumefaciens strains EHA 101 and EHA 105 contained the disarmed plasmid pCAMBIA 1304 which contains genes for hygromycin (hyg), β-gluconidase (gusA) and gfp (Fig. 2). The DHα E.coli strain ... flamborough women\\u0027s centre https://roderickconrad.com

A vacuolar phosphate transporter essential for phosphate ... - PNAS

WebClassification and research data for Binary vector pCAMBIA-1300, unclassified. Classification and research data for Binary vector pCAMBIA-1300, unclassified. Skip to … Webthe binary vector pCambia 1300-Rb:: uidA to replace the uidA gene and generate pCambia 1300-Rb::MCM2 fragment2. The M2-sense1 primer allowed us to add an XbaI restriction site at the 5 ′ end of the PCR product, and M2-antisense1 was designed downstream of the internal XbaI (1844 bp down-stream of the start codon) site. The … WebAug 14, 2015 · Grains of an insect resistant transgenic Bt rice (Oryza sativa L. Japonica cv Ariete), transformed by Agrobacterium tumefaciens with the binary vector pCAMBIA 1300 containing the synthetic Cry1B gene from Bacillus thuringiensis, and its corresponding wild-type (WT) were used in this study . The CRM of the GM maize MON863 9.85 % (ERM … flamborough whats on

Schematic representation of T-DNA region of pCAMBIA-1300 binary vector ...

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Binary vector pcambia-1300

Conferring DNA virus resistance with high …

http://www.biovector.net/product/1319.html WebApr 7, 2024 · How to order pCAMBIA 1300 vector? Question. 3 answers. Apr 3, 2024; ... The steps include cloning your gene into a binary vector, transferred the vector into Agrobacterium, ...

Binary vector pcambia-1300

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WebBinary vector pCAMBIA 1301 showing hptII gene near left border and GUS gene near right border of T-DNA from publication: An Efficient Protocol for the Agrobacterium-mediated Genetic Transformation ... WebJun 9, 2024 · In the first step, an EcoRI/KpnI fragment of ~ 2.4 kb was excised from the plasmid pKSMYMIV-A and cloned into the EcoRI/KpnI sites of binary vector pCAMBIA-1300 (Cambia) to produce the recombinant plasmid pCAM-MYMIV-AM.

WebJun 19, 2007 · Biological Sciences Two type III effector genes of Xanthomonas oryzae pv. oryzae control the induction of the host genes OsTFIIA γ 1 and OsTFX1 during bacterial blight of rice Akiko Sugio, Bing Yang, Tong Zhu, and Frank F. White Authors Info & … WebBinary vector pCAMBIA-1301 Taxonomy ID: 118389 (for references in articles please use NCBI:txid118389) current name. Binary vector pCAMBIA-1301. NCBI BLAST name: other sequences Rank: species Genetic code: Translation table 1 (Standard) Plastid genetic code: Translation table 11 (Bacterial, Archaeal and Plant Plastid)

WebThe cDNA was cloned into a binary vector (pCAMBIA 1300) and transformed into indica rice var Pusa Basmati-1. Southern hybridization analysis of transgenic rice plants revealed stable integration of the Sod1 transgene in the rice genome. The mRNA transcript of Sod1 was detected by Northern hybridisation in the transgenic rice plants. Web作为美国质粒载体菌株细胞株基因保藏中心分库及唯一办事处，Biovector Inc.可为您提供便捷一站式的产品进口服务，到货快捷，步骤简便。. 通过Google搜索"Biovector+您所需 …

WebBinary vector pCAMBIA-1300, complete sequence Binary vector pCAMBIA-1300, complete sequence. Your browsing activity is empty. Activity recording is turned off. Turn …

WebSep 8, 2014 · In this study, a novel binary T-vector was constructed by integrating two AhdI restriction sites into the backbone vector pCAMBIA 1300. The T-vector also contains a … can parkinson\u0027s disease be preventedWebSep 8, 2014 · In this study, a novel binary T-vector was constructed by integrating two AhdI restriction sites into the backbone vector pCAMBIA 1300. The T-vector also contains a … can parkinson\\u0027s disease be curedflamborough women\\u0027s resource centreWebNov 9, 2015 · For genetic complementation, the full-length genomic DNA of the VPT1 fragment was amplified from wild type genomic DNA and cloned into the binary vector pCAMBIA-1300. To generate the VPT1VPT1 coding sequence without a stop codon, and GFP (35S:VPT1-GFP) were cloned into the pCAMBIA-1300. The tonoplast marker, … can parkinson\u0027s come on suddenlyWebJun 1, 2024 · The new plant RNAi binary vector, named pCAMBIA2301-GW-RNAi, has two inverted repeated Gateway cassettes driven by the cauliflower mosaic virus 35S (CaMV … flamborough women\u0027s centreWebSep 16, 2008 · The first T-DNA of the pCAMBIA 1300 vector contained the hygromycin phosphotransferase (hpt) selectable marker gene, while the second T-DNA carried the β … can parkinson\u0027s disease cause back painWebThe pBIN19 vector was developed in the 1980s and is one of the first and most widely used binary vectors. The pGreen vector, which was developed in 2000, is a newer version of … can parkinson\u0027s cause dry mouth