Biotinylated hyaluronan binding protein

WebWe now report the preparation of biotinylated hyaluronic acid (molecular weight = 1.2 x 10 (6) Da) as a probe for histochemical and immunochemical characterization of HA-binding proteins. Approximately one-third of the available HA glucuronate residues could be readily biotinylated in high molecular weight HA. Publication types WebJan 1, 2002 · Growing evidence for intracellular hyaluronan in tissues in vivo, together with evidence of intracellular hyaluronan-binding molecules, prompted us to examine hyaluronan distribution and uptake, and hyaluronan-binding sites within cells and their relationship to cell proliferation in vitro using a biotinylated hyaluronan-binding protein …

Identification of Hyaluronic Acid-Binding Proteins and Their ...

WebApr 12, 2024 · Hyaluronan binding proteins are of two types; those with link modules, and those without. Link modules are 100 amino acid (aa) HA and proteinbinding sequences that contain two αhelices and two antiparallel βsheets (1, 3). There are three categories of link modulecontaining proteins. WebAug 8, 2024 · polyclonal antibody against CD44 has been described previ- ously (13). Biotinylated HA-binding protein (HABP) was pur- Cell proliferation in monolayers chased from Hokudo Co., Ltd. 4-Methylumbelliferone (4-MU) The cells (1,000 cells/well) were plated in growth medium in was purchased from Sigma-Aldrich. did matt brown attend billy brown funeral https://roderickconrad.com

Detection of Glycosaminoglycans in Pancreatic Islets and …

WebR.A. Kohanski, in Encyclopedia of Biological Chemistry (Second Edition), 2013 Abstract. The biotinylation of proteins is the covalent coupling of biotin to an amino acid or … WebFeb 3, 2024 · Abbreviations: biotinylated hyaluronan binding protein (bHABP), epidermis (ep), papillary dermis (pd), reticular dermis (rd). (C) Quantification of HA in the human and porcine skin. HA content in 100 µL of lysate from the epidermis and dermis was quantified via LC-MS/MS; the results were then adjusted for the initial tissue weight. WebJul 30, 2024 · Hyaluronan Binding Protein Assay. To localize hyaluronan (HA) in histologic sections, we performed a fluorescent HA binding protein assay ... After a rinse in PBS, biotinylated-HABP (1:100 in normal goat serum, Millipore-Sigma) was added to the slides for 1 h at room temperature. Next, the slides were washed with PBS 4 times and … did matt brown attend billy brown\u0027s funeral

Biotinylated hyaluronan: A versatile and highly sensitive probe …

Category:Biotinylated hyaluronan: A versatile and highly sensitive probe …

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Biotinylated hyaluronan binding protein

THE PRESENCE AND PROCESSING OF INTRACELLULAR HYALURONAN …

WebOct 1, 2002 · Identification of hyaluronan binding proteins using a biotinylated hyaluronan probe. Connect Tissue Res. 1993. 30: 117-126. Google Scholar. ... Webby the hyaluronan-binding protein. No correlation was found between the degree of HA deposition in the dermis and the expression of CD44 in the skin of shar-pei dogs affected or unaffected by cutaneous mucinosis. A clear positive correlation was found between the existence of clinical mucinosis and the serum HA concentration. In control dogs, serum

Biotinylated hyaluronan binding protein

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WebSep 6, 1996 · Link modules are hyaluronan-binding domains found in proteins involved in the assembly of extracellular matrix, cell adhesion, and migration. The solution structure of the Link module from human TSG-6 was determined and found to consist of two α helices and two antiparallel β sheets arranged around a large hydrophobic core. This defines the …

WebHyaluronan Bindling Protein (HABP), Versican G1 domain, bind specifically to Hyaluronan (HA) and doesn’t bind to other glycosaminoglycan and DNA, it can be used for HA … WebOct 1, 1999 · A biotinylated hyaluronan binding probe was used for localization of hyaluronan in human aortic smooth muscle cells and skin fibroblasts in vitro. In nonpermeabilized cells, hyaluronan staining was prominent on the cell surface and in the pericellular matrix as a tangled flocculent network ( Figure 1A ).

WebIn this chapter, we describe the preparation and use of b-PG, a biotinylated complex that specifically binds hyaluronan (1,2). The b-PG is derived from cartilage and consists of a … WebJan 21, 2024 · Biotinylated hyaluronan-binding protein; biotinylation of hyaluronan-binding protein was performed with biotin-aminocaproic acid sulfo-NHS (Uppsala, Sweden). Hermes-3 anti-CD44 antibody (kind gift from Professor Sirpa Jalkanen, University of Turku, Finland [ 10 , 11 ]).

WebFigure Legend Snippet: Hyaluronan-rich septa are largely unchanged throughout mammary gland development. Immunofluorescence microscopy for hyaluronic acid binding …

WebA convenient and reliable method for preparing and using biotinylated hyaluronan for detection of hyaluronan-binding proteins is described. The biotinylated hyaluronan … did matt carriker serve in the militaryWebIn this chapter, we describe the preparation and use of b-PG, a biotinylated complex that specifically binds hyaluronan (1,2). The b-PG is derived from cartilage and consists of a … did matt brown go to billy brown\u0027s funeralWebThe widespread occurrence of hyaluronan-binding proteins indicates that the recognition of hyaluronan is important to tissue organisation and the control of cellular behaviour. ... did matt brown go to his father\\u0027s funeralWebJan 31, 2011 · Knudson, CB; Knudson, W. Hyaluronan-binding proteins in development, tissue homeostasis, and disease. FASEB J 1993, 7 (13), 1233–1241. [Google Scholar] Weigel, PH; Hascall, VC; Tammi, M. Hyaluronan synthases. ... HA was detected using biotinylated HABP (d, e, f) as described previously . CD44 immunohistochemistry (g, h, … did matt brown attend fathers funeralWebApr 29, 2024 · Biotinylated hyaluronan binding protein (b-HABP) antibody (amsbio, AMS.HKD-BC41, 1:200 in antibody diluent) was added to the slides and incubated overnight at 4 °C. did matt brown go to his father\u0027s funeralWebGene expression by qRT-PCR was analyzed utilizing a panel of HA-related genes: hyaluronan synthesis ( Has1-3 ), hyaluronidases ( Hyal1,2 ), and hyaluronan receptors ( Cd44, Rhamm, Lyve1 ). Third and fourth mammary glands were pooled from 3–4 mice per sample (n). Error bars represent standard error of the mean. did matt brown go to billy\u0027s funeralWebHyaluronan (HA) is widely detected in biological samples and its concentration is most commonly determined by the use of a labeled specific HA binding protein (aggrecan G1 … did matt busby play for liverpool