WebJun 21, 2004 · Chk1 P-S345 and Chk2 P-T68 antibodies were obtained from Cell Signaling Technology (Beverly, MA). P-S1981 ATM antibody was from Rockland (Gilbertsville, PA). In Vitro Kinase Assays. Kinase assays were performed with Flag-tagged recombinant ATM or ATR proteins produced in human embryonic kidney (HEK) 293 cells as described in ref. 28. http://genesdev.cshlp.org/content/14/12/1448.full
Human Phospho-Chk1 (S345) Antibody AF2475: R&D …
WebJun 15, 2000 · We show that in human cells, Chk1 is phosphorylated on serine 345 (S345) in response to UV, IR, and hydroxyurea (HU). Overexpression of wild-type Atr enhances, … WebGeneral notes. Chk1 (phospho-Ser345) antibody detects endogenous levels of Chk1 only when phosphorylated at serine 345. The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines ... dustin proffitt
Phospho-CHK1 (Ser345) Monoclonal Antibody (S.48.4)
WebApr 29, 2009 · S. pombe Chk1 domain structure.Chk1 has an N-terminal kinase domain (blue) and a C-terminal regulatory domain (yellow). Regions required for 14-3-3 interaction and nuclear localization [] are indicated below the schematic.The position of S345 (orange), the site of activating phosphorylation is shown, as are the two regions of the C-terminal … WebJun 10, 2014 · Chk1 inhibition by V158411 in the leukemia and lymphoma cell lines induced DNA fragmentation and cell death that was both caspase dependent and independent, and prevented cells undergoing mitosis. ... was obtained from Millipore; Chk1, pChk1 (S317), pChk1 (S345), Cdc2, pCdc2 (Y15), Cyclin B1 and pH2AX (S139) from Cell Signaling … WebOct 18, 2024 · To detect P S317-Chk1, P S345-Chk1 and phosphorylation-induced mobility shifts of Chk2, pRb and p130, extracts were analyzed on 7.5% SDS-PAGE gels. Other cell cycle regulators and P T68 -Chk2 were ... dvd home theater receiver