Irf3 phosphorylation antibody

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August undefined, 2024

WebMar 6, 2012 · Aliquots of the cell lysates were analyzed by Western blotting with an antibody against IRF3 after native PAGE (top). Data are from a single experiment representative of … WebMar 12, 2015 · IRF3 activation requires phosphorylation, dimerization and nuclear translocation. However, the mechanisms for the termination of IRF3 activation in nucleus are elusive. Here we report the identification of TRIM26 to negatively regulate IFN-β production and antiviral response by targeting nuclear IRF3.

Anti-IRF3 Antibodies Invitrogen - Thermo Fisher Scientific

WebPhospho-IRF3 (Ser396) Antibody (MA5-14947) in WB Frontiers in microbiology 2024 - FIGURE 2 Effects of Set-Met on the expression of MAVS protein and the phosphorylation … WebApr 14, 2003 · Viral infection results in the phosphorylation of IRF3, leading to its dimerization and interaction with the coactivators CREB-binding protein (CBP) and p300 … grandma attacked outside store in edmond

DNA damage repair kinase DNA‐PK and cGAS synergize to induce …

WebOct 6, 2024 · IRF3 phosphorylation by TBK1 and IKKε leads to its dimerization and nuclear translocation, where it associates with CBP/p300 to initiate transcription of anti-viral target genes, such as type I interferons (TI-IFNs). TRAF3 plays a central role in Toll-like receptor (TLR) signaling through generation of activating polyubiquitin chains. ... WebKey features and details Rabbit polyclonal to IRF3 (phospho S396) Suitable for: WB Reacts with: Human Isotype: IgG You may also be interested in Primary Anti-IRF3 (phospho S386) … WebApr 11, 2024 · Since EhaF-mediated inhibition of TLR4-TRIF-IFN pathway occurs at the level of IRF3 phosphorylation ... Cells were lysed after 1.5 h and IRF3 was immunoprecipitated … chinese food long island city

A bacterial autotransporter impairs innate immune responses by ...

Pim1 promotes IFN-β production by interacting with IRF3

WebPhospho-IRF3 (Ser396) Antibody (MA5-14947) in WB Frontiers in microbiology 2024 - FIGURE 2 Effects of Set-Met on the expression of MAVS protein and the phosphorylation of IRF-3 induced by PDCoV. LLC-PK cells were incubated with PDCoV for 1 h, and 2, 4, 8, and 16 muM of Se-Met was added. WebPrimary Anti-IRF3 (phospho S386) antibody [EPR2346] - BSA and Azide free (ab182859) Protein Recombinant Human IRF3 protein (ab205216) View more associated products Overview Product name Anti-IRF3 (phospho S386) antibody [EPR2346] See all IRF3 primary antibodies Description Rabbit monoclonal [EPR2346] to IRF3 (phospho S386) Host … chinese food longmont coloradoWebThe induction of phosphorylation of STING on S366 of IRF3 on S396 was inhibited in T404E compared to WT or ... T404A, or T404E STAT2 were treated with cisplatin for 24 h. The cells were fixed then stained with IRF3 antibody (red) and DAPI for confocal analysis. (C) STAT2-deficient Calu-1 cells restored with WT, T404A, T404E, or Y690F STAT2 ... grandma attacked outside store in eugene

"Web), the data reported here suggest that additional signals stimulate phosphorylation of IRF3 and IRF7, essential events in induction of the protective IFN response. This evidence is … " - Irf3 phosphorylation antibody

Irf3 phosphorylation antibody

IRF3多克隆抗体 IRF3 Polyclonal Antibody-内参抗体-艾美捷科技有 …

WebMar 4, 2024 · IRF3 is a transcriptional factor that mediates expression of a large array of genes after viral infection. We hypothesized that DUBs might regulate the activity or stability of IRF3 and thereby facilitate or inhibit cellular antiviral responses. WebFigure Legend Snippet: TAOK1 promotes TBK1-IRF3 complex formation. a HEK293T cells were transfected with plasmids encoding HA-TBK1, Flag-IRF3, and varying doses of a plasmid encoding Myc-TAOK1 (0.5, 1.0, and 1.5 μg). Cells were harvested 24 h after transfection for immunoblot analysis as indicated with the antibody to Flag tag. b …

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WebInvitrogen Anti-Phospho-IRF3 (Ser396) Polyclonal, Catalog # 720012. Tested in Western Blot (WB) and Immunocytochemistry (ICC/IF) applications. This antibody reacts with Human … WebUse Anti-phospho-IRF-3 (Ser396) Antibody (Rabbit Polyclonal Antibody) validated in WB to detect phospho-IRF-3 (Ser396) also known as Interferon regulatory factor 3. Anti-phospho …

WebDec 15, 2014 · To directly observe the distribution of HSPD1 and activated IRF3, an antibody specific to phosphorylated IRF3 (phospho S386) was used. Not surprisingly, overexpression of MAVS for 16 h resulted in phosphorylation of IRF3. Furthermore, almost all of the activated IRF3 co-localized with HSPD1 at that time point . WebHere we show that STING stimulates IRF3 phosphorylation by the kinase TBK1 in an in vitro reconstitution system. Using this system, we identified a carboxyl terminal region of STING that is both necessary and sufficient to activate TBK1 and stimulate IRF3 phosphorylation.

WebOct 22, 2024 · Phosphorylation occurs when IRF3 is recruited to adaptor proteins via a positively charged surface within the regulatory domain of IRF3. This positively charged surface also plays a crucial role in forming the active homodimer by interacting with the phosphorylated sites stabilizing the homodimer. WebJan 19, 2024 · The phosphorylation of IRF3 plays critical roles in determining the immune state and the cell fate against viral infection. Until now, the sequential activation mode of IRF3 through multi-site phosphorylation remains controversial. ... Antibodies; Rabbit polyclonal anti-IRF3: Santa Cruz Biotechnology: Cat# sc-9082; RRID: AB_2264929: Anti …

WebAntibodies that detect IRF3 can be used in several scientific applications, including Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry and Peptide array. These antibodies target IRF3 in Human, Mouse, Rat, Non-human primate and Porcine samples. Our IRF3 monoclonal, polyclonal, recombinant monoclonal and recombinant ...

WebMar 21, 2024 · Entrez Gene Summary for IRF3 Gene. This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an … chinese food loomisWebSep 1, 2024 · Immunoblot analysis of IRF3 with anti-phospho-Ser82 antibody and IRF3 antibody in HEK293 cells untreated or infected with SeV (50 HA units/ml) for 8 hr, ... Phosphorylation on IRF3 could turn on or off its function, corresponding to positive or negative regulation, respectively. The positive and negative regulation phosphorylation, … grandma attacked outside store in folsomWeb该IRF3 Polyclonal Antibody. This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha … grandma at homeWebViral infection results in phosphorylation of IRF3, leading to its translocation to the nucleus, where it activates promoters containing IRF3-binding … chinese food longos plazaWebIRF3 and IRF7 Phosphorylation in Virus-infected Cells Does Not Require Double-stranded RNA-dependent Protein Kinase R or IκB Kinase but Is Blocked by Vaccinia Virus E3L Protein* - Journal of Biological Chemistry MECHANISMS OF SIGNAL TRANSDUCTION Volume 276, ISSUE 12, P8951-8957, March 2001 chinese food long neck delawareWebQing Yang, Hong-Bing Shu, in Advances in Immunology, 2024. 2.4.5 Regulation of IRF3 activity. Upon viral infection, IRF3 undergoes phosphorylation at S396 by TBK1, and … grandma attacked outside store in irvineWebNational Center for Biotechnology Information grandma attacked outside store in pomona